Immunoblots for K48 ubiquitin chain and g MGH protein modification in KOLF 2.1 J CTR ( n = 5), L166P-1 ( n = 6), and L166P-2 ( n = 3) (One-way ANOVA followed by Tukey’s for the multiple comparisons test). MGH protein modification in BJ-SIPS CTR ( n = 3) and KO ( n = 4) (Two-tailed t -test was used for mean comparisons). All data are represented in mean ± S.E.M., data points are individual well differentiation, and the p -value was reported on the graph highlighted comparison. Proteomics data related to KN073-96 dataset. Scale bars, 20 m. All measurements were taken from distinct samples.