Bar Plot from Scientific Research

Orthogonal R-loop assay to measure Cas9-independent off-target editing with the editing reagents used in this study. HEK293T cells were transfected with plasmids encoding nuclease-inactive S. aureus Cas9 and sgRNA targeting an established genomic test site that leads to high off-target editing when held in an open R-loop. After 24 h, cells were electroporated with ABE8e or ABE7.10 (as mRNA or protein) and sgRNA –175, followed by PCR and NGS to detect base edits. Frequencies of on-target –175A>G edit ( n = 3).

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