Bar Plot from Scientific Research

CD34 + HSPCs from two different healthy donors indicated by different symbols were treated with ABE RNPs containing sgRNA –175. After 6 days, the potential off-target editing sites identified by CIRCLE-seq and/or Cas-OFFinder (212 sites) were evaluated by multiplex PCR and NGS. Significant off-target editing was observed in three sites and one additional site approached significance. Editing is calculated as the number of reads with an A•T-to-G•C transition mutation within the editing window (nucleotides 4–10 of the protospacer alignment) divided by the total number of reads. Three replicates for each of two different donors ( n = 6) were quantified for on-target and off-target (OT) editing. Off-target base editing was observed after treatment with ABE8e, but not ABE7.10.

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