Gel from Scientific Research

Gel-shift assay results. COS cell extract expressing a FLAG tag fused to zinc fingers 4–6 of BCL11A was incubated with radiolabeled WT or mutant probes representing the γ-globin BCL11A binding motif, separated on a polyacrylamide gel and analyzed by autoradiography. Arrow shows the position of BCL11A bound to WT probe; asterisk, antibody-supershifted complex.

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