siRNA targeting WRN efficiently reduces the helicase expression level and promotes G4access signal at promoters. Left—representative western blots of total H3 and of WRN are shown ( n = 2 biological replicates; full blot scans of biological replicates are shown in Source data ). Right—DESeq volcano plot, performed on sequences with G4H > 1.2, result is displayed, (red) differential promoter signal, (blue) differential non-TSS signal, (gray) unaffected signals (DESeq, P < 0.05). Majority (97.8%) of differential promoters show an increased G4access signal (1,591/1,626; n = 2 biological replicates of G4access).
