Quantification of bundle thickness index versus time in assays performed as in (B), where red-colored letters denote experiments featuring the absence of the indicated proteins (“Z,” zyxin; “V,” VASP; “A,” α-actinin; “P,” profilin-G-actin). Data are presented as mean ± SEM; 7 ≤n≤ 11 trials from N= 2 biological replicates.