Microscopy from Scientific Research

Open access visualization of Microscopy, 3D Plot, LIONESS, Hippocampal Brain Slice, Axon Tracing
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Magnified view of three orthogonal planes from a LIONESS imaging volume in an organotypic hippocampal brain slice with example of a correctly traced axon (blue line with dots, manually generated in WebKnossos by a tracer blinded to the eGFP channel). Scale bar, 1 m. Position of subvolume relative to full imaging volume with two traced axons in red and blue (bottom right).

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