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Schematic comparison of LIONESS imaging with conventional STED imaging (based on the parameters used in restoration network training) in terms of light exposure and imaging time, as well as the reduction in segmentation time by automated over manual segmentation. The shading indicates that reduction in segmentation time by deep learning depends on sample complexity. LIONESS lookup tables are linear and inverted throughout, ranging from black (maximum photon counts extracellularly) to white.
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