Bar Plot from Scientific Research

Genome editing efficiencies using either iCRISPR–Cas9D10A double nicking, Cas9-HiFi RNP or Cpf1-Ultra RNP (Cas12a) in H9 hESCs that carry DNA-PKcs K3753R and transfected with the POLQ siRNAs (‘semitransient inhibition’, that is, genetic NHEJ inhibition, transient MMEJ inhibition). Frequencies of deletions are presented on the basis of microhomology length. Independent biological replicates were performed ( n = 2) and error bars show the s.e.m. For HDR, replicates are depicted by dots. The panel to the right gives mean outcome purities (percentage HDR of all editing events) for each target. Each dot indicates the mean of one target, the box the 25th to 75th percentile, lines medians and whiskers extend from minimum to maximum values.

DOI