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The bar charts, illustrating cases 1-4, show query versus reference database alignment scores ranked in decreasing order. Taxon information of the best hit is assigned to each query protein together with a confidence score (proportional to dot size). Protein-wise taxon call examples: Case 1: many hits support the metazoa (green) taxon label that is assigned with a high confidence score. Case 2: bacteria (purple) taxon label is assigned but due to limited support, the confidence score is lower. Case 3: Fungi label (blue) from a single hit is assigned albeit with a very low confidence score. Case 4: No hit observed for query in the reference database.

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