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Benchmarking of nonbleaching nanoscopy: fluorescence super-resolution versus PINE. (i) Time t = 0 s: Diffraction-limited darkfield image (top left) and PINE-resolved image (bottom left) at time t = 0 s. Diffraction-limited fluorescence image (top right) and fluorescence super-resolution resolved image (bottom right) at time t = 0 s. Scale bar: 200 nm. (ii) Time t = 200 s: Diffraction-limited darkfield image (top left) and PINE-resolved image (bottom left) at time t = 200 s. Diffraction-limited fluorescence image (top right) and fluorescence super-resolution resolved image (bottom right) at time t = 200 s. Scale bar: 200 nm. (iii) Graph of average scattering intensity over time for fluorescence super-resolution versus PINE. PINE (purple), fluorescence super-resolution (green).
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