The reversal potentials ( V rev ) of channel currents. The symbols (black for the wild types, red for the Y81A mutants, blue for the Y106A mutants and green for the Y106F mutants; filled for the peak currents and empty for the end currents) are the mean values, the error bars, s.e.m ( n = 8 cells for Hc KCR1_Y106F and WT Hc CCR, and 7 cells for other variants). p = 0.02, 0.03 and 0.06 (peak currents) and 0.002, 0.04, and 0.01 (end currents) for Hc KCR1_Y81A, Y106A and Y106A, respectively; 0.82, 0.08 and 0.04 (peak currents) and 1, 0.45, and 0.1 (end currents) for Hc CCR_Y81A, Y106A, and Y106A, respectively by the two-sided Mann-Whitney test compared to the respective WT. n.s., not significant. Source data are provided as a Source Data file.
