The reversal potentials (V rev ) of channel currents measured in the variants with Ile, Ser or Thr at the residue position 73. Wild-type Hc KCR1 contains Ile, and wild-type Hc CCR, Ser at this position. The symbols (black for the I mutants, red for the S mutants and blue for the T mutants; filled for the peak currents and empty for the end currents) are the mean values, the error bars, s.e.m. (n = 8 cells for Hc KCR1_I73S and WT Hc CCR, and 7 cells for other variants). p = 2.97E-07 (peak currents) and 5.20E-09 (end currents) by one-way ANOVA with the Tukey correction for multiple comparisons (alpha 0.0001 for Hc KCR1 currents and 0.05 for Hc CCR currents) to the respective I variants. n.s., not significant. Source data are provided as a Source Data file.