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A Robertsonian translocation in the lineage leading to E. coqui (Eco), shown compared with E. pustulosus (Epu) and X. tropicalis; The analyzed species were visualized with a custom script, alignment_plots.py (v1.0, https://github.com/abmudd/Assembly ). For each plot, the Hi-C inference-based centromeric regions are depicted with black stars, the X. tropicalis centromeric satellite repeat from tandem repeat analysis with a red star (on X. tropicalis chromosomes 7 and 1 (a, b), the stars overlap), the density of L1 repeats per chromosome with gold densities, and the runs of collinearity containing at least one kilobase of aligned sequence between the species with connecting black lines. kb kilobases, Mb megabases. Source data are provided as a Source Data file.

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