Boxplots for the distribution of the maximum probability of assigning a cell to a layer (left panel; L1: n = 11,807 cells; L2: n = 15,745 cells; L3: n = 15,949 cells; L4: n = 14,121 cells; L5: n = 13,332 cells; L6: n = 25,107 cells; WM: n = 22,988 cells) and of assigning a cell to a layer by cell types (middle panel; Ast: n = 12,126 cells; End: n = 444 cells; Ex: n = 39,176 cells; In: n = 12,286 cells; Mic: n = 3,982 cells; Oli: n = 44,182 cells; Opc: n = 6,853 cells). In each boxplot, the lower and upper hinges correspond to the first and third quartiles, and the center refers to the median value. The upper (lower) whiskers extend from the hinge to the largest (smallest) value no further (at most) than the 1.5 x interquartile range from the hinge. Data beyond the end of the whiskers are plotted individually. Layers or cell types that have higher mapping certainty will show higher maximum probabilities. Proportions of cells that are mapped to each layer by cell types (right panel). L1–L6 and WM were defined similarly as in Fig. 4b. Ast astrocyte, End endothelial, Ex excitatory neuron, In an inhibitory neuron, Mic microglia, Oli oligodendrocyte, Opc oligodendrocyte progenitor cell.