IFN protein in BAL from ( b ) male and ( c ) female resistant (PWK, NZO and 129S1 x K18-hACE2) and sensitive (K18-hACE2 and A/J x K18-hACE2) mice at 3 and 6 dpi. Biological replicates were examined over at least 2 independent experiments and the numbers of mice assessed per strain (male: female) were as follows: 3 dpi (K18-hACE2 (5:7); A/J x K18-hACE2 (5:5); PWK x K18-hACE2 (7:6); NZO x K18-hACE2 (7:7); 129S1 x K18-hACE2 (6:5); CAST x K18-hACE2 (8:6); NOD x K18-hACE2 (5:4); WSB x K18-hACE2 (6:9), and 6 dpi (K18-hACE2 (6:6); A/J x K18-hACE2 (6:7); PWK x K18-hACE2 (6:7); NZO x K18-hACE2 (7:5); 129S1 x K18-hACE2 (5:5); CAST x K18-hACE2 (8:9); NOD x K18-hACE2 (4:6); WSB x K18-hACE2 (5:7). Two-tailed Student t -test with 95% confidence interval was used to compare cytokine production in ( a ) male versus female for each strain, ( b ) resistant versus sensitive males, and ( c ) resistant versus sensitive females. * p < 0.05 was considered statistically significant.
