Representative fluorescence images of rhodamine- (red) and DAPI- (blue) stained HUASMCs showing that the (rhCol III/PDA-PEI) n coatings loaded with different amounts of rhCol III all suppressed HUASMCs compared with the control PLA group. Scale bars, 200 m. Quantification of ( b ) cell number and ( c ) cell viability of HUVSMCs cultured on uncoated- and (rhCol III/PDA-PEI) n -coated PLA sheets ( n = 1, 2, and 4) after 1 day and 3 days of culture ( n = 5 independent samples). d Volcano plot showing differentially expressed genes in the (rhCol III/PDA-PEI) 2 group compared to the PLA control group. Downregulated and upregulated genes were colored blue and red, respectively, at significantly differentially expressed thresholds log 2 FC > 1.5 and p < 0.05. e GO analysis of differentially expressed genes in HUASMCs. f Heatmap of the differentially expressed genes in the PLA and (rhCol III/PDA-PEI) 2 groups. PCR arrays displaying the expression of genes related to the ( g ) synthetic phenotype, ( h ) contractile phenotype, and ( i ) relaxation of HUASMCs in the PLA and (rhCol III/PDA-PEI) 2 groups. The value in the (rhCol III/PDA-PEI) 2 group was normalized to that in the PLA group ( n = 3 independent samples). Two-way ANOVA with Tukeys multiple comparisons was used for the comparisons in ( b ), ( c ), and ( g )–( i ). Two-sided Students t test with multiple testing corrections was used in ( d ) and ( e ). The data are presented as the mean SD ( p values < 0.05 were considered statistically significant, #### indicated p values < 0.0001 compared with other groups).
