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A maximum likelihood phylogenetic tree of phnJ genes from phycospheric heterotrophic bacterial bins and cyanobacteria. A total of 128 phnJ genes from 647 phycospheric heterotrophic bacterial bins and 151 cyanobacterial phnJ genes [ 25 ] were used in tree construction. Clades selected for the design of primer set are shown as red ( Pseudomonadales ), green ( Rhodospirillales ), and blue ( Rhizobiales ) branches. Rings from outer to inner are (1) the relative abundance of each phycospheric bacterial bin in each strain sequencing project, (2) bins generated from Microcystis sequencing, and (3) the taxonomic classification of cyanobacterial or heterotrophic bacterial strain for each phnJ gene
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