Precision-recall graphs of MetaPro, HUMAnN3, and SAMSA2s gene annotation strengths against the gold standard hits. True positives are annotations of reads from the pipeline that agree with the gold standard. True negatives are reads that are unidentified by both the pipeline and the gold standard. False negatives are reads that the gold-standard identified but were not annotated by the pipeline. False positives are when the pipeline annotated a read where the gold standard did not. Due to the low number of true positives across all samples and all tools, precision and recall are low, and the plots do not resemble a typical precision-recall plot.