Line Plot from Scientific Research

Flow cytometry of PGAP1-KO cells expressing hPGAP1 Jelly-roll or cPGAP1 Jelly-roll treated with (orange) or without (cyan) PI-PLC using CD59 as the GPI-AP reporter with gating strategies provided in Fig. S12a. PIGK-KO cells, in which GPI-AP biosynthesis are blocked 46, 57, are used as a staining control (gray). Typical results of three independent experiments are shown. Source data of (e) are provided as a Source Data file. EM electron microscopy, KO knockout.

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