Bar Plot from Scientific Research

The RNA cleavage efficiency of CasRx on endogenous genes. HEK293T cells were co-transfected with CasRx-P2A-mCherry and gRNA plasmids, and mCherry + cells were sorted out by FACS for RNA extraction and quantitative RT-PCR analyses. n = 3 independent experiments. NT, Non-targeting gRNA, which recognized no site in the human genome and transcriptome. For d , ** p <0.01, *** p <0.001, Student’s t test

DOI