Illustration from Scientific Research

Open access visualization of Illustration, Table Image, gRNAs-directed DNA cleavage, Lb Cas12a, HEK293T cell line
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DOI: 10.1186/s13059-023-02992-z

DOI

The efficiency and specificity of different gRNAs-directed DNA cleavage at 14 sites in a Lb Cas12a knock-in HEK293T cell line revealed by Tag-seq. The gRNA reference as well as the on-target and off-target sites was shown on the left, and sequencing read counts were shown to the right of each site.

#Illustration#Table Image#gRNAs-directed DNA cleavage#Lb Cas12a#HEK293T cell line#Tag-seq#On-target sites#Off-target sites#Sequencing read counts

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