Flow cytometry analysis of cytokines in CD8+ T cells after 72 h of treatment. T cells were isolated from spleens of naive C57BL/6 mice and treated with vehicle or recombinant adiponectin in the presence of anti-CD3/CD28 beads. Data from two experiments. Data shown as mean ± SEM. Normality assessment: Shapiro-Wilk test. Significance testing: Welch’s ANOVA with Dunnett’s multiple comparison (A), RM one-way ANOVA with Tukey’s multiple comparison (D and E), unpaired Student’s t test (J and Q), one-way ANOVA with Tukey’s multiple comparison (K and M), Kruskal-Wallis test with Dunn’s multiple comparison (N and O), Welch’s corrected unpaired Student’s t test (P) or paired Student’s t test (S, T, and U). See also Figures S10 and S11.
