Nile red preferentially stains fat body and Uranine preferentially stains hemolymph. For honey bee pupa (Pup, n = 6 replicates, 1 pupa/replicate) only the abdomen was collected for analysis. For protonymphs (Pro), deutonymphs (Deu), and foundresses (Fou) of V. destructor mites, six biological replicates were prepared with each replicate containing three individual mites of the same development stage (n = 6 replicates, 3 mites/replicate). For mite feces (Fec) samples, also six replicates were prepared, and in each replicate feces from three capsules were collected and pooled together (n = 6 replicates, 3 capsules/replicate). Absolute fluorescence intensities were log-transformed. All data are presented as mean ± S.D. Source data are provided in the Source Data file.
