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Antibacterial activity via determination of Colony-forming unit (CFU) reduction of bacteria after treatment with protein nanospears for 1 h at 37 °C. The short-dashed line represents a level of no colony detection.pvalues were calculated using one-way ANOVA followed by comparison between the treatment groups and respective controls (*p< 0.1, **p< 0.01, ***p< 0.001, and ****p< 0.0001). The experiment was performed in at least three biological replicates on different days. The error bars represent the standard deviation (SD) of the mean.
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