Workflow from Scientific Research

A diffraction-limited image is convolved with a top-hat Gaussian kernel, which is then intensity thresholded to produce a binary mask that is then dilated and eroded to produce a mask with the aggregates and aggregate-bound liposomes manifesting as bright connected regions. These regions are segmented and their integrated intensities (Is) and count (C) are calculated to infer the calcium influx and concentration of aggregates in a sample of interest.

DOI