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Open access visualization of Microscopy, Experimental Scheme, Bright-field Image, Immunofluorescence, POU5F1
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Experimental scheme depicting the conditions used to disrupt the lumens at day 2 (top), associated bright-field image at day 2 after dissociation, and immunofluorescence for POU5F1, SOX17, and TFAP2C at day 5. Dashed box is magnified (right), showing separated channels. Scale bars: 50 μm. See alsoFigure S3.

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