Analysis of PhTx effects in current clamp recordings (muscle 6 NMJs, 0.2 mM extracellular Ca2+) of AP-evoked synaptic activity in wild-type (w1118) animals with prior PMA treatment. Left: representative AP-evoked eEPSP responses (average of five repetitions in one cell) of NMJs preincubated for 10 min with 2 uM PMA (+PMA) followed by 10 min incubation either with (+PhTx, 20 uM) or without (-PhTx) philanthotoxin. Middle and right: quantification of eEPSP amplitude and quantal content. Number of cells (n) and animals (N) investigated: n/N(-PMA; -PhTx) = 15/8, n/N(-PMA; +PhTx) = 17/9, n/N(+PMA; -PhTx) = 14/7, n/N(+PMA; +PhTx) = 17/9. Genotype (seeSTAR Methodsfor details):w1118wild type. Statistical analysis with Mann-Whitney U test. Mean values +/- SEM are indicated in plots; n.s., p > 0.05;*p <= 0.05;**p <= 0.01;***p <= 0.001. Source data available inTable S6.
