Same as (A)–(D), but for NMJs stained with an Unc13A antibody recognizing its C-terminal MUN domain. SeeFigure S4for an independent analysis that evaluated protein distribution in the entire AZ. Genotypes (seeSTAR Methodsfor details): Unc13A and -B null animals expressing either wild-type Unc13A (Ctrl) or theUnc13ACaMWRWRmutant (Unc13A CaMWRWR). Number of analyzed AZs (ν), NMJs (n), and animals (N): Unc13A N-Term staining, side-view AZs, ν/n/N(Unc13CaMWRWR) = 85/10/5; ν/n/N(Ctrl) = 63/10/5; top-view AZs, ν/n/N(Unc13CaMWRWR) = 72/10/5; ν/n/N(Ctrl) = 49/10/5; Unc13A C-Term staining, side-view AZs, ν/n/N(Unc13CaMWRWR) = 75/7/4; ν/n/N (Ctrl) = 104/9/4; top view AZs, ν/n/N(Unc13CaMWRWR) = 126/7/4; ν/n/N(Ctrl) = 128/9/4. Scale bars: 400 nm. Data depict the mean values ± SEM. Statistical analysis of line profiles with repeated measures two-way ANOVA, comparison of ring diameters (B and C) and distances from BRP peaks (F and G) with unpaired parametric t tests; ns, p > 0.05;*p ≤ 0.05;**p ≤ 0.01. Source data available inTable S3.
