Averages across repeated locomotion bouts for standard deviations (SDs) of neural activity over time for prior 2-s temporal window (n = 116, five mice, mean SEM). Time courses were aligned around locomotion onset (left) and locomotion offset (right). Inset brain maps on (D) depict the SD of neural activity 6–8 s before and 5–7 s after locomotion onset, and the p values and z values of the comparison between the SDs (Wilcoxon rank-sum test, p < 0.05, Bonferroni corrected).Zscores set to zero for statistically insignificant comparisons. Note that sensory hindpaw and visual regions show a substantial increase in averaged moving-window SD during locomotion onset simply because signals are rapidly increasing on a single trial level.