Box plots showing all SNVs tested within MPRA mutagenesis experiments, their significance and their effects on expression. The bounds of the box plots define the 25th, 50th and 75th percentiles, and whiskers are 1.5x the interquartile range. The y axis is the log of the adjusted P value (P adj ) with the direction of expression change (positive values indicate an increase in expression and negative values indicate a decrease in expression). Dashed horizontal lines indicate significance thresholds at P = .05. Each dot represents a tested SNV. SNVs that have a significant increase in expression are shown in green, those with no significant change in expression are grey and those with a significant decrease in expression are red. For each plot, we show SNVs that increase the affinity of the transcription factor, SNVs within the TFBS that do not alter affinity and all other SNVs. Analysis of the ETS TFBS in a saturation mutagenesis MPRA on a 485-bp region of the ZRS enhancer.
