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PI4KII GST-fusion proteins generated from either the FL PI4KII N terminus (WT) or versions with phosphomimic (S47/51D) or -null (S47/51A) substitutions were incubated with nerve terminal lysates, and the presence of either BRSK1, CAMKV, AGAP2, TRIM3, or STOP175 was determined by western blotting.
#Illustration#PI4KII#GST-fusion proteins#Nerve terminal lysates#Western blotting#BRSK1#CAMKV#AGAP2#TRIM3#STOP175
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