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Detection of SRSF3 and SRSF3-TR in whole-cell extracts from 293T cells exogenously expressing FLAG-tagged full-length (FL) or truncated (TR) SRSF3 compared with protein lysates from MB135-iDUX4 myoblasts treated with (+) or without (-) doxycycline (Dox) to induce DUX4 and immunoprecipitated with a custom anti-SRSF3-TR antibody, no antibody (Ab), or a commercial SRSF3 antibody. IP, immunoprecipitation. Asterisks denote proteins of interest. Note the FLAG-tagged versions of SRSF3-FL and SRSF3-TR run at a higher molecular weight than the endogenous versions due to the epitope tag. Representative image of two biological replicates shown.

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