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Absorbance at 254 nm across a sucrose density gradient of lysates from control MB135-iDUX4 myoblasts and MB135-iDUX4 myoblasts expressing DUX4 for 14 h (top left, n = 1). qRT-PCR measurement of RPL27, SRSF3NMD+, and SRSF3NMD mRNA levels in DUX4-expressing myoblasts relative to control myoblasts from collected fractions (remaining panels, n = 3 technical replicates).

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