Microscopy from Scientific Research

High-magnification confocal microscopy reveals perinuclear TrkB clusters associated with Lamp-1-positive structures and SORCS-2 in the absence of DRD1 activity. Stimulation of DRD1 for 10 min causes TrkB-IR at cell membrane positions, less association with Lamp-1 structures, and SORCS-2 at perinuclear sites. Blockade of lysosomal acidification and degradation by bafilomycin results in huge perinuclear TrkB cluster formation, association with Lamp-1-positive structures, and significant overlap with a SORCS-2 cluster at the same position.

DOI