Line Plot from Scientific Research

Representative Ultra-High-Performance Liquid Chromatography-diode array detection (UHPLC-DAD)-chromatograms recorded at 450 nm of extraplastidial carotenoids inNicotianaleaves agro-infiltrated with constructs encoding AL-1, AL-2, and AL-3 (AL-1/-2/-3), tHMGR, AL-1, AL-2, and AL-3 (tHMGR/AL-1/-2/-3), or with the empty vector (EV, control). P1 (peak 1), -carotene; P2 (peak 2), lycopene; P3 (peak 3), torulene; P4 (peak 4), 3-4 didehydrolycopene. The insets next to the chromatograms display the Ultraviolet-visible (UV-vis) spectra of these four carotenoids. The images show representativeNicotianaleaves agro-infiltrated with EV, AL-1/-2/-3, or tHMGR/AL-1/-2/-3. The Eppendorf tubes next to the leaves contain the corresponding non-polar extracts.

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