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Graphs report the frequency of embryos with GFP expression in CHT ECs after injection with wild-type sequences or mutated variants of the mrc1a 125 bp enhancer. Data is normalized to the wild-type control (44% [155/356]). Mean ± SEM, one-way ANOVA with Dunnett’s multiple comparisons test;***p < 0.001,****p < 0.0001. All experiments were performed at least three times, with independent clutches.
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