Flow cytometry of S. aureus cells harbouring various pCRISPR constructs and stained with DiOC 2 (3), collected 30 min after addition of aTc or the depolarizing agent CCCP. a.u., arbitrary units; green fluorescence, emission of 515 nm upon 488 nm excitation; red fluorescence, emission of 610 nm upon 561 nm excitation. Each plot is representative of approximately 100,000 cells. Blue points represent low density of events while red points represent higher density of events.
