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Normalized anti-AstC signals in Epi neurons. The brains were incubated at room temperature (∼24°C), at 39°C, or at 44°C for 5 min. n = 10–16 neurons from ≥5 dissected brains. Error bars indicate SEMs. Mann-Whitney test.∗∗p < 0.01; ∗∗∗p < 0.001.
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