Microscopy from Scientific Research

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Representative images of organoids after treatment with DMSO (vehicle control) and alpelisib (100 μM). EOC1120_pOme1 represents the control organoid line with no alteration in the PI3K pathway and EOC989_r1Asc represents an organoid line with enriched PI3K activity. After drug treatment, the total number of nuclei were stained with Hoechst 33342 (blue) and the dead cells with propidium iodide (PI; red). Images were acquired under 10× magnification with ImageXpress Confocal HT.ai microscope. Scale bar, 50 μm.
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