Quantification of image-based cell proliferation of organoids after treatment with 50 μM alpelisib, idelalisib, or umbralisib and DMSO for 48 h. Graphs represent the estimated percentage of Ki67 positive nuclei out of the total number of Hoechst 33852-positive nuclei followed by normalization to the DMSO control values (DMSO values were set as 100% proliferation). Data are shown as a pool of three experiments done as triplicates and presented as mean ± SEM. Statistical significance was calculated using one-way ANOVA combined with Dunnett’s multiple comparisons to the negative control. In all cases, p > 0.05,*p < 0.05,**p < 0.01,***p < 0.001, and****p < 0.0001. See alsoTable S11.
