Workflow from Scientific Research

Schematic illustration of the experimental layout used to obtain data shown in (B)–(K). In brief, brains with macroscopic CT-2A-Ova tumors were collected on day 14 post-tumor implantation from AAV-GFP- or AAV-LIGHT-treated mice (n = 2 mice/group) for single-cell RNA sequencing (scRNA-seq) analysis. A trajectory analysis of CD8+T cell differentiation from the Tstem-likestate to the Texhaustedstate was created using the set of genes listed inTable S5. The data were later validated by flow cytometry.

DOI