Diagram of known protein motifs of DAR4 and mutant and chimeric forms used to test hypersensitive responses. TIR, Toll, and interleukin-1 receptor homology regions; NB, NB-ARC nucleotide-binding; ARC, APAF-1,R proteins, and CED-4 domains; LRR, leucine-rich-repeat domain; LIM, LIN11, Isl1, and MEC3 domains; each Zn finger of the two LIM domains is represented; PEP, zinc-metallopeptidase region with AHEMMHA-EE active site; GST, glutathione S-transferase; GFP, green fluorescent protein. Red letters indicate the positions of mutated amino acid codons. C1340Y is in the fourth Zn-finger of the LIM repeats. H1488, H1492, E1508, and E1509 are in the HEMMA and EE motifs of the peptidase active site. The diagram is not to scale.Figure S2shows the amino acid sequences and motifs of the DA1 family.
