Illustration from Scientific Research

Insertion sites of Tn6022 and ZooTsy on E. coli endogenous comM and comM of their respective hosts used in the pTarget. ComM protein sequences (translated comM) are shown below the nucleotide sequence. The pink rectangle indicates the genomic location of the Walker B of the AAA ATPase encoded by comM; the red rectangle shows the probable hot spot binding region of both TnsFs.

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