Microscopy from Scientific Research

Cells described in (A) and (B) were grown on glass cover slips overnight and incubated in growth medium (DMEM) or starvation medium (EBSS) for 1 and 3 h. Imaging was performed using a Zeiss Axio Observer 7 fluorescence microscope equipped with a 40x/1.4 Oil DIC M27 Plan-Apochromat objective and ApoTome 2. Representative sections are depicted. Scale bars: 20 m. The ratio of puncta to cell nuclei of 20 images of three independent experiments is depicted in an interleaved box and whiskers plot (Tukey style). At least 500 cells per condition were analyzed. See alsoFigures S3S5.

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