Schematic (top) and quantification (bottom) of IFN Lucia reporter activity in RAW 264.7 macrophages after treatment with supernatant from control pMMECs (NT) or pMMECs expressing the indicated sgRNAs. Graph depicts mean s.e.m., n = 3 (NT, sgControl, sg Mre11 , sg Mlkl ) samples from 3 independent biological experiments. Comparisons by two-tailed t -tests, NT versus sgControl, P < 0.0001; sgControl versus sg Mre11 , P < 0.0001; sgControl versus sg Mlkl , P = 0.0007.
