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Cell-cycle score. A cycling score combining the expression of S- and M-phase characteristic genes (seeSTAR Methods) was assigned to each single cell (y axis). Due to the low number of PGCs in the manifold, cells were binned to four groups (6–6.6, 7–7.4, 7.5–7.9, 8.1–8.6). Partial cycling slowdown in the PGC population is observed, when compared with the notochord (substantially halting) or other early populations (fully cycling).
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