Images (k) and quantification (l) FRAP assays in sgControl versus si MRE11 cells stably expressing GFPcGAS. Images taken at 1-min intervals before and after photobleaching an arbitrary nuclear region of interest (ROI) for 60 min. Neocarzinostatin (NCS; 0.1 mg ml 1 ) was added immediately after photobleaching as indicated. n = 3 independent cells for each condition. Two-tailed two-way ANOVA: siControl + NCS versus si MRE11 + NCS, P = 0.041; siControl versus siControl + NCS, P = 0.0003; siControl + NCS versus si MRE11 , P < 0.0001. Scale bars, 10 m
