Images (m) and quantification (n) of colocalization of cGAS-ISD90 in cGAS-deficient control or sg MRE11 cells reconstituted with cGAS WT or cGAS(R255A). White arrowheads indicate colocalization of cGAS and ISD90 foci. Data are mean s.e.m., n = 1,200 independent cells per condition. sg cGAS + cGAS WT + doxycycline versus sg cGAS /sg MRE11 + cGAS WT + doxycycline, P < 0.0001; sg cGAS + cGAS(R255A) + doxycycline versus sg cGAS /sg MRE11 + cGAS(R255A) + doxycycline, P > 0.99 (NS). Unless otherwise specified, grouped analyses performed with a two-tailed t -test. Scale bars, 10 m
