Illustration from Scientific Research

Schematic of split GFP-based AWC-AIY NLG-1 GRASP marker. Circles represent cross-sections of the AWC and AIY neurites, and one neurite from each neuron pair is represented for simplicity. Split GFP fragments are linked to the pre- and post-synaptically localized protein NLG-1 (neuroligin 1) and expressed in the AWC and AIY neurons with the selective promoters podr-1 and pttx-3. When synapses form between the neurons, the split GFPs come in contact, reconstitute, and fluoresce. White circles indicate a presynaptic site, and crosshatching represents a post-synaptic site.

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